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KMID : 0613820080180040503
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Journal of Life Science
2008 Volume.18 No. 4 p.503 ~ p.508
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Effect of Deep Sea Water on Cytochrome P450 1A1, Aromatase and MMP-9
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Shon Yun-Hee
Kim Mee-Kyung
Nam Kyung-Soo
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Abstract
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Deep sea water from the East sea was tested for breast cancer chemoprevention and metastasis by measuring the activities of cytochrome P450 1A1 and aromatase, invasiveness, and activity and expression of matrix metalloproteinase (MMP)-9 in breast MDA-MB-231 cancer cell. The in vitro incubation of rat liver microsome with deep sea water (a hardness range of ) showed a hardness-dependent inhibition of 7,12-dimethylbenz[a]anthracene (DMBA)-induced cytochrome P450 1A1 activity. Deep sea water showed 27.1, 45.4 and 51.9% inhibition of microsomal aromatase activity at the hardness of 600, 800 and 1,000, respectively. In addition deep sea water inhibited not only the invasiveness of 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MDA-MB-231 cells through matrigel-coated membrane in a hardness-dependent manner but also the activity and expression of MMP-9 in MDA-MB-231 cell.
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KEYWORD
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Deep sea water, cytochrome P450 1A1, aromatase, matrix metalloproteinase, breast cancer chemoprevention
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